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Open Access Article

Biomedical Engineering and Biomechanics. 2025; 1: (1) ; 14-19 ; DOI: 10.12208/j.beb.20250002.

Characterizing Severe Early Childhood Caries Microbiota through Culturomics
通过培养组学表征儿童早期严重龋齿的微生物群

作者: Xiaowei Zhao *, Yuhong Li

湖北省口腔医学基础科学国家重点实验室培育基地暨口腔生物医学教育部重点实验室(武汉大学) 湖北武汉

*通讯作者: Xiaowei Zhao,单位:湖北省口腔医学基础科学国家重点实验室培育基地暨口腔生物医学教育部重点实验室(武汉大学) 湖北武汉;

发布时间: 2025-04-24 总浏览量: 91

摘要

本研究纳入了两名SECC患儿和两名同龄无龋儿童,利用培养组学技术,即通过设定不同的条件(温度、氧气条件、培养基)对口腔牙菌斑中的微生物进行分离培养,通过16S rRNA测序鉴定菌种,并结合临床信息对菌株功能进行深入分析。本研究共鉴定出37种菌种,其中4种以前从未与口腔相关,3种从未从临床标本中分离过。这项先导研究能够为SECC患者和同龄健康儿童的菌群复杂性提供一些新的信息。

关键词: SECC;培养组学;口腔微生物群

Abstract

Severe early childhood caries (SECC) is a common widespread caries affecting multiple deciduous teeth in children, which seriously affects children’s physical and mental health and growth. Investigation of their pathogenic flora is essential. Two children with SECC and two caries-free children of the same age were included in this study. Using cultureomics techniques, that is, microorganisms in oral dental plaque were isolated and cultured by setting different conditions (temperature, oxygen conditions, culture medium). Species were identified by 16S rRNA sequencing, and strain function was analyzed in depth in combination with clinical information. A total of 37 species were identified in this study. 4 species had never been associated with the oral cavity before, and 3 of them had never been isolated from clinical specimens. This pilot study was able to provide some new information on the complexity of the microbiota in SECC patients versus healthy children of the same age.

Key words: SECC; Cultureomics; Oral microbiota

参考文献 References

[1] Li K, Wang J, Du N, Sun Y, Sun Q, Yin W, Li H, Meng L, Liu X. Salivary microbiome and metabolome analysis of severe early childhood caries. BMC Oral Health. 2023 Jan 19; 23(1):30. 

[2] Cvanova M, Ruzicka F, Kukletova M, Lipovy B, Gachova D, Izakovicova Holla L, Danek Z, Hola V, Bartosova M, Jarkovsky J, Dusek L, Borilova Linhartova P. Candida species and selected behavioral factors co-associated with severe early childhood caries: Case-control study. Front Cell Infect Microbiol. 2022 Jul 25; 12:943480.

[3] Peng X, Cheng L, You Y, Tang C, Ren B, Li Y, Xu X, Zhou X. Oral microbiota in human systematic diseases. Int J Oral Sci. 2022 Mar 2; 14(1):14. 

[4] Pathak JL, Yan Y, Zhang Q, Wang L, Ge L. The role of oral microbiome in respiratory health and diseases. Respir Med. 2021 Aug-Sep; 185:106475. 

[5] Matar G, Bilen M. Culturomics, a potential approach paving the way toward bacteriotherapy. Curr Opin Microbiol. 2022 Oct; 69:102194. 

[6] Lagier JC, Dubourg G, Million M, Cadoret F, Bilen M, Fenollar F, Levasseur A, Rolain JM, Fournier PE, Raoult D. Culturing the human microbiota and culturomics. Nat Rev Microbiol. 2018 May 1; 16:540-550. 

[7] Hamad I, Ranque S, Azhar EI, Yasir M, Jiman-Fatani AA, Tissot-Dupont H, Raoult D, Bittar F. Culturomics and Amplicon-based Metagenomic Approaches for the Study of Fungal Population in Human Gut Microbiota. Sci Rep. 2017 Dec 1; 7(1):16788. 

[8] Li Y, Kawamura Y, Fujiwara N, Naka T, Liu H, Huang X, Kobayashi K, Ezaki T. Rothia aeria sp. nov., Rhodococcus baikonurensis sp. nov. and Arthrobacter russicus sp. nov., isolated from air in the Russian space laboratory Mir. Int J Syst Evol Microbiol. 2004 May; 54(Pt 3):827-835. 

[9] Jia YJ, Liao Y, He YQ, Zheng MQ, Tong XT, Xue WQ, Zhang JB, Yuan LL, Zhang WL, Jia WH. Association Between Oral Microbiota and Cigarette Smoking in the Chinese Population. Front Cell Infect Microbiol. 2021 May 28; 11:658203. 

[10] Ortiz S, Herrman E, Lyashenko C, Purcell A, Raslan K, Khor B, Snow M, Forsyth A, Choi D, Maier T, Machida CA. Sex-specific differences in the salivary microbiome of caries-active children. J Oral Microbiol. 2019 Aug 28; 11(1):1653124. doi: 10.1080/20002297.2019.1653124. PMID: 31497256; PMCID: PMC6720314.

[11] Harris-Ricardo J, Fang L, Herrera-Herrera A, Fortich-Mesa N, Olier-Castillo D, Cavanzo-Rojas D, González-Quintero R. Bacterial profile of the supragingival dental biofilm in children with deciduous and early mixed dentition using next generation sequencing (HOMINGS) technique. Enferm Infecc Microbiol Clin (Engl Ed). 2019 Aug-Sep; 37(7):448-453. English, Spanish. 

[12] Callaghan MM, Dillard JP. Mucus Is a Key Factor in Neisseria meningitidis Commensalism. mSphere. 2019 Dec. 4; 4(6): e00777-19. 

[13] Zhang G, Rudney JD. Streptococcus cristatus attenuates Fusobacterium nucleatum-induced cytokine expression by influencing pathways converging on nuclear factor-κB. Mol Oral Microbiol. 2011 Apr; 26(2):150-63.

[14] Treerat P, Redanz U, Redanz S, Giacaman RA, Merritt J, Kreth J. Synergism between Corynebacterium and Streptococcus sanguinis reveals new interactions between oral commensals. ISME J. 2020 May; 14(5):1154-1169.

[15] Yesilkaya H, Oggioni MR, Andrew PW. Streptococcus pneumoniae: 'captain of the men of death' and financial burden. Microbiology (Reading). 2022 Dec; 168(12). 

[16] Tabah A, Laupland KB. Update on Staphylococcus aureus bacteraemia. Curr Opin Crit Care. 2022 Oct 1; 28(5):495-504.

[17] Atiencia-Carrera MB, Cabezas-Mera FS, Vizuete K, Debut A, Tejera E, Machado A. Evaluation of the biofilm life cycle between Candida albicans and Candida tropicalis. Front Cell Infect Microbiol. 2022 Aug 18; 12:953168. 

[18] Al-Lozi A, Cai S, Chen X, Perez VL, Venkateswaran N. Granulicatella Adiacens as an Unusual Cause of Microbial Keratitis and Endophthalmitis: A Case Series and Literature Review. Ocul Immunol Inflamm. 2022 Jul; 30(5):1181-1185. 

[19] Padilla Bautista M, Farrais Villalba M, Hayek Peraza M. Atypical presentation of infective endocarditis due to Granulicatella adiacens. Med Clin (Barc). 2022 Dec 20:S0025-7753(22)00589-9. English, Spanish.

[20] Shirazi MSR, Al-Alo KZK, Al-Yasiri MH, Lateef ZM, Ghasemian A. Microbiome Dysbiosis and Predominant Bacterial Species as Human Cancer Biomarkers. J Gastrointest Cancer. 2020 Sep; 51(3):725-728.

[21] Siqueira JF Jr, Rôças IN. Catonella morbi and Granulicatella adiacens: new species in endodontic infections. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006 Aug; 102(2):259-64.

[22] Khan M, Djamei A. Performing Infection Assays of Sporisorium reilianum f. sp. Zeae in Maize. Methods Mol Biol. 2022; 2494: 291-298. 

[23] Dai C, Hou Y, Xu H, Umego EC, Huang L, He R, Ma H. Identification of a thermophilic protease-producing strain and its application in solid-state fermentation of soybean meal. J Sci Food Agric. 2022 Apr; 102(6):2359-2370. 

[24] Thorat V, Kirdat K, Tiwarekar B, Dhanavade P, Karodi P, Shouche Y, Sathe S, Lodha T, Yadav A. Paenibacillus albicereus sp. nov. and Niallia alba sp. nov., isolated from digestive syrup. Arch Microbiol. 2022 Jan 8; 204(2):127. 

[25] Dürre P. Physiology and Sporulation in Clostridium. Microbiol Spectr. 2014 Aug; 2(4):TBS-0010-2012. 

[26] Grier A, Myers JA, O'Connor TG, Quivey RG, Gill SR, Kopycka-Kedzierawski DT. Oral Microbiota Composition Predicts Early Childhood Caries Onset. J Dent Res. 2021 Jun; 100(6):599-607. 

[27] Pilarczyk-Zurek M, Sitkiewicz I, Koziel J. The Clinical View on Streptococcus anginosus Group - Opportunistic Pathogens Coming Out of Hiding. Front Microbiol. 2022 Jul 8; 13:956677. 

[28] Issa E, Salloum T, Tokajian S. From Normal Flora to Brain Abscesses: A Review of Streptococcus intermedius. Front Microbiol. 2020 May 7; 11:826. 

[29] Tan DJA, Sng ECY, Ling XW. Clostridium septicum brain abscess. BMJ Case Rep. 2022 Nov 21; 15(11):e251719. 

[30] Fessia AS, Odierno LM. Potential factors involved in the early pathogenesis of Streptococcus uberis mastitis: a review. Folia Microbiol (Praha). 2021 Aug; 66(4):509-523.

引用本文

XiaoweiZhao, YuhongLi, 通过培养组学表征儿童早期严重龋齿的微生物群[J]. 生物医学工程与生物力学, 2025; 1: (1) : 14-19.